| 초록 |
We report the construction of a novel and efficient platform metabolic pathway for the bio-based production of four-carbon (butyrolactam), five-carbon (valerolactam), and six-carbon lactams (caprolactam). This pathway uses ω-amino acids as precursors and comprises two steps. Activation of ω-amino acids catalyzed by the Clostridium propionicum β-alanine CoA transferase (Act) followed by spontaneous cyclization and the novel pathway was validated both in vitro and in vivo. Three metabolically engineered Escherichia coli strains were developed by introducing the newly constructed metabolic pathway followed by systems-level optimization, which resulted in the production of butyrolactam, valerolactam, and caprolactam from the renewable carbon source. In particular, fed-batch fermentation of the final engineered E. coli strain produced 54.14 g/L of butyrolactam from glucose using the minimal medium. These results demonstrate the high efficiency of the novel lactam pathway developed in this study. |
