| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
[생물공학] 바이오팩토리 합성 최신현황과 차세대응용 |
| 제목 |
장내미생물 공배양을 위한 대장 모델 개발 Development of an in vitro human colon model system for gut bacterial coculture |
| 초록 |
Interactions of gut microbes with their host have significant impacts on the health and disease states of the host. Understanding these interactions can lead to discovering new diagnostic tools and therapeutic targets. An in vitro gut bacterial co-culture model system with human colon cells can be a valuable tool for testing hypotheses on gut microbes/host interactions and screening probiotics or pathogens with much less cost than animal models. However, creating a physiological oxygen gradient necessary for survivals of human cells and gut bacteria, primarily anaerobic, within one system has been challenging. We developed a novel and simple in vitro model system with a physiological oxygen gradient using cellular respiration and modulating oxygen influx. Multiple anaerobic gut bacteria were successfully co-cultured with the human colon epithelial cells. This platform provides a valuable platform for investigating and further utilizing the interactions of gut bacteria with human cells. |
| 저자 |
김래현1, Peter J. Attayek2, Kathleen L. Furtado2, Rita Tamayo2, Nancy L. Allbritton3
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| 소속 |
1홍익대, 2Univ. of North Carolina, 3Universit of Washingon |
| 키워드 |
colon; gut microbes; coculture; oxygen gradient
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| E-Mail |
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