| 초록 |
In this study, we report a novel platform that facilitate the spontaneous formation multicellular spheroids and precise real-time assessments of the anticancer effects of curcumin in a brain tumor model. A highly conductive gold nanostructure (HCGN) was developed to generate cancer spheroid formation. A neuroblastoma (SH-SY5Y) and glioblastoma (U-87MG) co-culture model was formed on HCGN with a specific cell-to-cell ratio (1:1). Using electrochemical signals as an indicator of spheroid viability, the effects of anticancer compounds were further measured. Interestingly, decreased cell viability in spheroids treated with a curcumin (30 µM) was detectable using the electrochemical method (29.4 %) but not with a conventional colorimetric assay (CCK-8). The electrochemical detection was sequentially done more than ten times for both short- (63 hours) and long-term cultivation (144 hours), enabling real-time and non-destructive pharmacokinetic analysis of various drug candidates. |
