| 초록 |
To investigate cell-cell interaction and barrier functions, organ chips integrated with various porous membranes have been used for co-culture. However, these porous membranes have limited mechanical characteristics, which are low porosity, poor light transmittance, and low permeability without any cell polarity control. In this study, we present how to fabricate a highly ordered polylactic-co-glycolic acid (PLGA) microfibers membrane with high permeability to control cell polarity. Using Nearfield Electrospinning technique, about 3.14 ± 0.31 µm PLGA fibers (10 % w/w Hexafluoro-2-propanol) were formed and printed on a PEO coated Si wafer to create rectilinear grid. The following layers were created after rotating in +/- 45° to fabricate a multilayered membrane with ~13 µm pore size. This membrane then embedded into two chamber PDMS microfluidic chip. We confirmed that myofibroblasts were cultured in the chip to observe how the cells can proliferate and migrate through the fibers. With high controllability on fiber size, pore size, and permeability, this PLGA membrane embedded organ chip enables us to mimic various barrier functions to study cell-cell communication and interaction. |
