| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Development of an in vitro co-culture device for the investigation of host–microbe interactions through LC-MS/MS based multiomics approaches |
| 초록 |
Akkermansia muciniphila is one of the next-generation probiotics for promoting human health. But the use of it as a cell therapy requires studying the molecular-level mechanisms of how it affects human intestinal epithelial cells. Here, we developed a Mimetic Intestinal Host-Microbe Interaction Coculture System (MIMICS) suitable for multiomics studies. And the physiological changes of intestinal epithelial cells due to co-culture with active A. muciniphila were analyzed. First, it was confirmed that the physiological activities of the two cells were stably maintained. Second, the membrane integrity of Caco-2 cells is maintained and up-regulation of tight junction proteins. Third, Caco-2 cells induced enhancement of energy production through activation of lipid metabolism. Consequently, we propose a novel mechanism for effects caused by A. muciniphila and MIMICS will be very useful for studies investigating the effects of specific gut microbiota at the molecular level. |
| 저자 |
권지은, 김윤곤, 조성현, 박지현, 김예림, 김민규, 백지현, 권서영
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| 소속 |
숭실대 |
| 키워드 |
Akkermansia muciniphila; Host–microbe interaction; Multiomics analysis
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| E-Mail |
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