| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생체재료 |
| 제목 |
Nanoassembly of chemically modified Cas9 ribonucleoprotein for in vivo gene editing and cancer immunotherapy |
| 초록 |
Gene therapy using clustered regular interspaced short palindromic repeats (CRISPR) system is challenging because of poor delivery and efficacy in vivo. We report the development of a nano-assembled ribonucleoprotein complex (NanoRNP) that can efficiently block the PD-L1 immune checkpoint in vivo and induce anti-tumor effects. Chemically modified CRISPR-associated protein 9 (Cas9) with single guide RNA and modified DNA oligonucleotide formed a stabilized NanoRNP. Delivery of NanoRNP in vivo in a mouse melanoma model can induce indels in tumor, thus significantly inhibiting tumor growth without including combination therapy. Blockade of the PD-L1 checkpoint by NanoRNP in tumor promotes suppression of immunosuppressive myeloid cells, as well as T cell infiltration and effector cytokine release, which are hallmarks of antitumor immune activation. This system proposes a promising strategy as an in vivo gene editing platform for cancer immunotherapy. |
| 저자 |
이주희, 정현정
|
| 소속 |
한국과학기술원 |
| 키워드 |
CRISPR; gene editing; immunotherapy
|
| E-Mail |
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