| 초록 |
3D cell spheroids incorporating biomaterials have been widely studied due to the advantage of being able to control the functionality of cells within the spheroid by direct delivery of instructive signals. Recently, 3D artificial adipose tissue for tissue reconstruction and drug screening has been highlighted. However, fabrication of 3D adipose tissue has been limited due to the use of mature adipocytes with low viability and the requirement of complex components (eg, IBMX, indomethacin, dexamethasone, insulin) for adipogenic differentiation. Here, we engineered adipogenic nanofibers (AF) to internally deliver adipogenic signals to 3T3-L1 (pre-adipocytes) within spheroids. AF incorporated 3T3-L1 spheroids enhanced adipogenic gene expression and formation of lipid droplets. Then, we encapsulated AF spheroids in Gel-MA hydrogels to generate 3D adipose tissue constructs. 3T3-L1 cells actively spread in the hydrogel and their maturation was induced by adipogenic signals from AF. |
