| 초록 |
We fabricated a novel transparent biodegradable thin film containing a cell-derived matrix for the human corneal endothelial cell (hCEC) transplantation. The film consists of a poly (lactide-co-caprolactone) (PLCL) physically bound to decellularized ECM (UMDM) derived from cord blood-derived mesenchymal stem cells. This transparent 20 μm thick ECM-PLCL film has a comparable elastic modulus to that of the human cornea, and UMDM contains abundant fibronectin, type IV collagen, and growth factors. The hCEC showed excellent adhesion and maintenance of hexagonal shape, formatting tight cell-cell junctions and sodium-potassium ion channels. The film was implanted to the anterior chamber of the rabbit eye for 8 weeks to assess the safety. The corneal thickness, cell density, and morphology were maintained normal status. Our study demonstrates that ECM-PLCL film could provide CECs with outstanding microenvironment and thus may serve as a novel CECs carrier toward corneal endothelium recovery. |
