| 학회 |
한국화학공학회 |
| 학술대회 |
2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 |
28권 1호, p.1005 |
| 발표분야 |
[주제 12] 화학공학일반(부문위원회 발표) |
| 제목 |
Yeast-derived Vacuoles as a Novel Carrier with Enhanced Blood–Brain Barrier Penetration for Targeted Neurodegenerative Therapy |
| 초록 |
The blood–brain barrier (BBB) is a brain protection structure that restricts drug delivery from the blood to the central nervous system. Thus, we developed a novel drug carrier using yeast vacuoles to overcome this problem. This study aimed to assess the drug transportability of yeast vacuoles using an in vitro BBB model. We used daunorubicin (DNR) as a microtubule-targeting agent with the ability to disaggregate pre-formed fibrils and prevent Tau fibrillization. The in vitro model of the BBB was developed using the human cerebral microvascular endothelial cell line (hCMEC/D3). The yeast vacuole was loaded with DNR, and the signals inside and outside the BBB model were detected. Confocal imaging showed a significant increase in intracellular DNR fluorescence when the cells were treated with the vacuole-encapsulated drug. These results indicate that the drug penetrated the BBB via encapsulation into the vacuoles. Yeast-derived vacuoles are promising candidates as drug carriers to the brain. This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (NRF-2021R1A2C2093580). |
| 저자 |
응웬티눅한1, 민지호2
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| 소속 |
1전북대, 2Department of Bioprocess Engineering |
| 키워드 |
생물화공(Biochemical Engineering) |
| E-Mail |
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| 원문파일 |
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