| 초록 |
Glycosylation is the common modification that diversify flavonoids with eminence biological activities. Amentoflavone, a biflavonoid is a dimer of apigenin exhibiting varied pharmaceutical properties. In this study, a glycosyltransferase, OleD GT was exploited for the glycosylation of amentoflavone in vitro. The OleD GT was functionally expressed in E. coli and employed for the glycosylation of amentoflavone via the transfer of glucose moiety from UDP-glucose. As a result, amentoflavone monoglycoside was evident in HPLC and LC-ESI-mass spectrometry. Furthermore, the glycosylation reaction conditions were optimized for maximum conversion at 30 min. This study proves that amentoflavone was successfully converted to corresponding monoglycoside. Based on the above results, it was concluded that OleD GT could catalyze the transfer of a glucose molecule to hydroxyl group of biflavonoids to produce amentoflavone monoglycoside with maximum conversion up to 85%. |
