| 학회 |
한국재료학회 |
| 학술대회 |
2021년 가을 (11/24 ~ 11/26, 경주 라한호텔) |
| 권호 |
27권 2호 |
| 발표분야 |
B. 나노화학/바이오 재료 분과 |
| 제목 |
Synthesis and biocompatibility of allogeneic extracellular matrix for tissue regeneration |
| 초록 |
Extracellular matrix (ECM) contains proteins and growth factors necessary for cell adhesion, proliferation and intercellular communication. This group of proteins plays a vital role for tissue regeneration by providing homing effect to cells. Decellularized extra cellular matrix bears the same potentials as the least amount of native components and growth factors remain intact. Whole organ ECM and ECM derived products accompanied with biocompatible polymers can be good substitutes for tissue regeneration. Due to supportive micro environment and preserved biochemical information, stem cells could be rehabilitated and differentiated into this ECM. There are some polymers that could mimic the ECM environment for cells and widely used for cell delivery in tissue engineering. Among them, sodium alginate is the most renowned polymer generally derived from sea weeds. Its’ contribution on tissue engineering like substitute fabrication, cell encapsulation or drug loading is knew no bound. However the main limitation of alginate is that although it behaves like ECM, it has no cell binding motif as ECM. Especially arginylglycylaspartic (RGD) motif is responsible for cell attachment with ECM. In this present study, attempts had been taken to utilise porcine liver derived ECM to chemically tag these RGD motifs with alginate chain. The decellularized ECM not only contained RGD motif, but also some growth factors and bio active proteins. The total ECM was digested to make smaller segments and peptide chains. These smaller peptide segments were attached with alginate by Schiff base reaction scheme. It was interestingly observed that the cellular proliferation rate was increasing after tagging and it was quite proportional to the concentrations of ECM used to modify alginate. The alginate group that contained higher amount ECM, showed progressive incremental growth of cells with time. The modified alginate was suitable for either mat or gel fabrication |
| 저자 |
Md. Sohanur Rahaman1, Byong-Taek Lee2
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| 소속 |
1Institute of Tissue Regeneration, 2Department of Regenerative Medicine |
| 키워드 |
ECM; Decellularization; Recellularization; Kidney; Bone; Regeneration; Modification
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| E-Mail |
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