| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 |
16권 2호, p.1520 |
| 발표분야 |
생물화공 |
| 제목 |
Purification and characterization of lignin peroxidase from a versatile mushroom Sparassis crispa and screening their degradation property |
| 초록 |
Peroxidases are oxidoreductases enzyme present in number of microorganisms and plants. Sparassis crispa, commonly called Ggotsongyi in Korean. In the present study we performed different experimental procedures for the determination of various peroxidases like laccase, lignin and manganese from this mushroom. Peroxidase activity was monitored by spectrophotomeric method as well as by the microtiter plate assay. The assay mixture consists of 80 mM pyrogallol, 100 mM sodium acetate buffer pH 5.4 and 80 mM hydrogen peroxide. Lignin peroxidase was measured with two assays: quantitation of the veratraldehyde produced on oxidation of veratryl alcohol. The assay was also used to monitor oxidation of the dye azure B involving absorbance measurements in the visible range. Application study was demonstrated by the enzymatic degradation of carbon nanotubes using the S.crispa lignin peroxidase. We summarize the potential application of this fungal peroxidase in chemical industry, coal chemistry and environmental production, etc. |
| 저자 |
Gayathri Chandrasekaran, 신현재, 남형근, 김윤수
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| 소속 |
조선대 |
| 키워드 |
Sparassis crispa; peroxidase
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| E-Mail |
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| 원문파일 |
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