| 초록 |
Effective therapeutic approaches using near infrared (NIR) irradiation (artificial light source) have been used for cosmetic and clinical dermal therapy. However, some issues about the deleterious effects of NIR have been raised because of the rise in skin temperature caused by NIR irradiation. As a result, research efforts have been very limited for developing NIR therapeutics to activate the proliferation of human keratinocytes and collagen synthesis in the skin microenvironment, and the subject has not been fully explored. Herein we describe a novel synthesis of Ag2S nanoparticles (NPs) by using a sonochemistry method and the effects of NIR irradiation on enhancement of the production of collagen through NIR-emitting Ag2S NPs. We also synthesized Li-doped Ag2S NPs that exhibited significantly enhanced emission intensity from their enhanced absorption ability in the UV - NIR region. Both Ag2S and Li-doped Ag2S NPs activated the proliferation of HaCaT skin fibroblast cells with no effect on cell morphology. In addition, Ag2S NPs activated TGFβ1 signaling by increased phosphorylation of Smad2 and Smad3. Especially, the degree of activation was more eminent in Li-doped Ag2S NPs, which was mainly because of higher PL intensity from Li-doping of Ag2S NPs. Our results suggest that the newly synthesized Ag2S NPs emit NIR and the Li-doping procedure elevates PL intensity. NIR emission by Ag2S NPs activates cell proliferation and collagen synthesis in skin keratinocytes. This efficacy has an applicable potential for clinical light therapy and the development of anti-wrinkle agents in cosmetics. |
