| 학회 |
한국재료학회 |
| 학술대회 |
2020년 가을 (11/18 ~ 11/20, 휘닉스 제주 섭지코지) |
| 권호 |
26권 1호 |
| 발표분야 |
B. 나노화학/바이오 재료 분과 |
| 제목 |
PCR-coupled Surface-enhacned Raman Scattering (SERS) system for Rapid and Sensitive Respiratory Bacterial DNA Detection |
| 초록 |
A paper-based silver nanowire surface-enhanced Raman scattering (SERS) substrate was developed and integrated with polymerase chain reaction (PCR) for rapid and sensitive detection of respiratory bacterial DNA. The gene employed in this study for demonstrating the applicability of the proposed approach is Mycoplasma Pneumoniae (M. Pne). A DNA intercalating molecule, i.e., EVA Green dye, was introduced to the low-cycled PCR product and the difference in the dye intercalation in the DNA structure was measured and quantified by Raman spectroscopy to verify the presence of the target gene. The detection capability of conventional fluorescence-based real-time PCR (RT-PCR) and the PCR-coupled SERS methods at various gene concentrations and amplification cycles were compared to develop a detection system capable of low-cycle amplification and sensitive DNA sensing. It was observed that the PCR-coupled SERS method exhibits enhanced detection capability with a detection limit of 3.12 pg/μL. Then, a SERS rapid kit was prepared by 3D printer and their diagnostic performance was demonstrated using the target DNA after 10 cycles of amplification. Compared to a non-target control, it successfully discriminated the presence of foreign gene diagnosing the infection. The developed system is expected to be integrated with diverse point-of-care (POC) diagnostic platforms for various bacterial and viral analysis. |
| 저자 |
Hyo Geun Lee1, Seung Yun Yang2, Sung-Gyu Park3, Min-Young Lee4, Ho Sang Jung5
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| 소속 |
1Advanced Nano-Surface Department, 2Korea Institute of Materials Science (KIMS), 3Department of Biomaterials Science, 4Life and Industry Convergence Institute, 5Pusan National Univ. |
| 키워드 |
<P>Bacterial DNA detection; Surface Enhanced Raman Scattering; Point-of-Care test(POCT); Molecular Diagnostics</P>
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| E-Mail |
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