| 학회 |
한국고분자학회 |
| 학술대회 |
2021년 가을 (10/20 ~ 10/22, 경주컨벤션센터) |
| 권호 |
46권 2호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Cell mimicking nanoparticles for rapid detection of highly pathogenic and low pathogenic avian influenza viruses via viral fusion mechanism |
| 초록 |
Avian influenza virus (AIV) causes acute infectious diseases of poultry, especially High pathogenic AIV (HPAIV) are cause of morbidity and mortality. Therefore, it is very important to take preventive measures through rapid detection of HPAIV for minimizing economic loss. In this study, we developed cell mimicking nanoparticles (CMPs) for a rapid detection of HPAIV and LPAIV via viral fusion mechanism. CMPs was polymeric nanoparticles constructed with sialic acid and FRET dye pairs, exposing FRET off signal responding to LPAIV and HPAIV activated by enzymatic cleavage at endosomal condition. CMPs exhibited accurate detection of various types of LPAIVs and HPAIVs in both cases with egg-cultured and fecal spiked specimen, showing superior sensitivity compared to rapid kit. The proposed strategy for mimicking viral infection can be a potent diagnostic assay for LPAIV and HPAIV, contributing to minimize economic loss by execution of preventative measures against the viral outbreaks. |
| 저자 |
박근선1, 임종우1, 박채원1, 염민주2, 함승주1
|
| 소속 |
1연세대, 2고려대 |
| 키워드 |
Cell-mimetic nanoparticles; rapid detection; avian influenza; viral fusion
|
| E-Mail |
|
