| 초록 |
Exosomes are cup-shaped lipid bilayer vesicles, ranging in size from 30-150 nm, which are secreted into body fluids by various cells in living organisms in the form of cytosol, and belong to one of the extracellular vesicles. As the research progresses, exosomes are used in a wide range of applications, such as early cancer markers and targeted drug carriers. How to isolate exosomes from body fluids rapidly and non-destructively is the difficulty faced at present. Traditional ultracentrifugation, polymer precipitation, and microfluidic techniques have various drawbacks, so to achieve high throughput, high recovery, and non-destructive separation of exosomes, by using the advantages of mesoporous silica with controllable pore size, pore length, and orientation. Mesoporous silica films with vertical pore channels were designed. The functionalized Mesoporous Silica Film (FMSF) was synthesized by a simple functionalization of the film to achieve reusability, while the exosomes in complex body fluid samples were rapidly separated by a size exclusion method. |
