| 학회 |
한국고분자학회 |
| 학술대회 |
2021년 가을 (10/20 ~ 10/22, 경주컨벤션센터) |
| 권호 |
46권 2호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Structure Specific Enzymatic Sensor for Universal Viral Nucleic Acid Detection via Surface-Enhanced Raman Spectroscopy (SERS) |
| 초록 |
Viral nucleic acids are a valuable source for disease detection since it encodes every characteristic of a virus. This study presents a system in which flap endonuclease 1 (FEN1) is used to spot and quantify viral cDNA in a sensitive and swift manner. FEN1 is known to remove 5’ overhanging DNA flaps depending on specific double-flap structures. Synthetic DNA strands according to random cDNA fragments of viral mRNA target sequence forms a double-flap structure with two kinds of additive oligonucleotides, enabling recognition by FEN1: Raman dye labeled probe that is partially complementary and forms a 5’-flap, and another sequence providing 3’-1nt flap. FEN1 removes the Raman dye labeled 5’-flap from the double-flap substrate which is used to trace the presence of target viral gene sequence by SERS. This system detecting any gene with considerably high speed and sensitivity can lead to a step forward to preemptive prevention of widespread viral infectious diseases. |
| 저자 |
박주원1, 김진영1, 함승주1, 박채원1, 김은정2, 염민주3
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| 소속 |
1연세대, 2인천대, 3고려대 |
| 키워드 |
FEN1; vRNA; nucleic acid; SERS; universal nucleic acid sensor
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| E-Mail |
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