| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 가을 (10/23 ~ 10/24, 부산 BEXCO) |
| 권호 |
14권 2호, p.2758 |
| 발표분야 |
생물화공 |
| 제목 |
Global analysis of protein localization in Escherichia coli B |
| 초록 |
Recently, the full genome sequences of two representative descendants of E. coli B, REL606 and BL21(DE3), have been completely determined by International E. coli B Consortium. Here we systematically determined for the first time the subcellular localizations of E. coli B REL606 by analyzing cytoplasmic, periplasmic, inner and outer membrane, and extracellular proteins based on the genome information using two-dimensional gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). This study represents one of the large-scale proteomic analyses of the subproteome of E. coli B. [This work was supported by the Korean Systems Biology Research Grant from the Ministry of Education, Science and Technology through the Korea Science and Engineering Foundation (No. M10309020000-03B5002-00000).]. |
| 저자 |
한미정1, 이정욱2, 윤홍석3, 김지만1, 이상엽2, 유종신3
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| 소속 |
1Metabolic and Biomolecular Engineering National Research Laboratory, 2Department of Chemical & Biomolecular Engineering and BioProcess Engineering Research Center, 3KAIST |
| 키워드 |
Escherichia coli B; two-dimensional gel electrophoresis; proteome profiling; subcellular localization
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| E-Mail |
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| 원문파일 |
초록 보기 |
