| 학회 | 한국화학공학회 |
| 학술대회 | 2011년 가을 (10/26 ~ 10/28, 송도컨벤시아) |
| 권호 | 17권 2호, p.1496 |
| 발표분야 | 생물화공 |
| 제목 | Anti-obesity effect by Hizikia fusiformis Extracts treated with the complex enzymes |
| 초록 | The object of this study were to evaluate the anti-obese effect of Hizikia fusiformis. An active inhibitor was purified from Hizikia fusiformis Extracts treated with the complex enzymes (;Viscozyme, celluclast, Alcalase). The purified inhibitor was identified by thin-layer chromatography, infrared and nuclear magnetic resonance spectroscopy. Hizikia fusiformiscompetitively inhibited lipase activities using emulsified triolein and dispersed 4-methylumbelliferyl oleate (4-MU oleate) as substrates. Lipase activity was also measured using 4-MU oleate as a substrate. The reaction was started by adding lipase (TypeVI-S:fromporicine pancrease).After incubation at 37°C for 30min. The amount of 4-methylumbelliferone released by the lipase was measured fluorometrically at an excitation wavelength of 360 nm and an emission wavelength of 460 nm. |
| 저자 | 남보미, 이중헌 |
| 소속 | 조선대 |
| 키워드 | anti-obesity; hizikia fusiformis; complex enzyme |
| 원문파일 | 초록 보기 |
