| 학회 | 한국화학공학회 |
| 학술대회 | 2016년 가을 (10/19 ~ 10/21, 대전컨벤션센터) |
| 권호 | 22권 2호, p.1795 |
| 발표분야 | 생물화공 |
| 제목 | Single amino acid replacement transforms mCherry to a far-red fluorescent protein |
| 초록 | Far-red fluorescent proteins are beneficial for imaging in mammals. Here, starting from mCherry, the most commonly used among the different types of red fluorescent proteins (RFP), not having a H-bond network in its original form, we sought to recover the hydrogen bond network in mCherry. By comparing the structure of avGFP and mCherry, we focused on a few key residues involved in a proton wire, and discovered an I197T mutant that showed a more red-shifted fluorescence. The detailed optical and photo-switching properties of related engineered RFPs are described. This study will guide further development of monomeric far-red FPs. |
| 저자 | 김예지1, 송경주1, 이화진1, 김도현2, 김진태3, 정민섭1 |
| 소속 | 1홍익대, 2명지대, 3건국대 |
| 키워드 | 효소/단백질공학; 바이오칩/나노바이오공학 |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
