| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 |
19권 1호 |
| 발표분야 |
고분자_포스터 |
| 제목 |
Development of target molecule detection and capture system utilizing fluorogenic molecular net; polymer based DNA hydrogel |
| 초록 |
We propose fluorometric sensor system for target molecule capture/detection utilizing polymer based DNA hydrogel (PD-hydrogel). The fluorescent PD-hydrogel composed by acrylamide, bis-acrylamide and Acryd-DNA was prepared by radical polymerization and applied to detect target; DNA mismatch and small molecule as ATP. Through introducing fluorophore modified competitor DNA (Fl-cpDNA), target capture/detection utilizing PD-hydrogel was confirmed by both fluorescence decrement of PD-hydrogel and fluorescence increment of remained solution since the Fl-cpDNA in PD-hydrogel were detached and released into the remained solution. Our developed PD-hydrogel platform would be easily applied to detection and capture of target selectively, by fluorogenic method. |
| 저자 |
이지현, 김원종
|
| 소속 |
POSTECH |
| 키워드 |
DNA; hydrogel; DNA polymer hydrogel; capture; sensor
|
| E-Mail |
|
