| 초록 |
Fluorescence has become a widely used tool in life science today, such as biological imaging and sensing. Although Fluorescence is a highly sensitive technology, the detection of the fluorophore are limited by their quantum yields (brightness) and photo stabilities. In this work, we have developed core-double shell structure (silver core-silica and Albumin shell) nanoparticles with an optimized shell thickness featuring europium (Eu3+) –tetracycline (Tc) fluorescence complex (EuTc) for Tc detection and cell imaging. The core-double shell architecture (AgNP@SiO2@BSA) has demonstrated its applicability in fluorescence signal amplifying technology termed metal-enhanced fluorescence (MEF). MEF is an established technology, where the interactions between excited states of fluorophores and the induced surface plasmons of metal nanoparticles results in fluorescence enhancement, increased photostability, decreased lifetime, reduced blinking in single molecule fluorescence spectroscopy, and increased transfer distances for fluorescence resonance energy transfer. |
