| 초록 |
A biodegradable polyurethane has been developed using L-tyrosine (LTU2a). This polymer was synthesized using desaminotrosyl tyrosine hexyl ester, polyethylene glycol (PEG), and 1,6-diisocyantohexane (HDI). LTU2a was designed to degrade through hydrolysis and enzymatic mechanisms since the backbone of the polymer contained a peptide bond. Using water-in-oil-in-water emulsion, nanoparticles encapsulating complexes of DNA and linear polyethylenimine LPEI or bovine serum albumin labeled with fluorescein have been fabricated. In addition, their surfaces have been decorated with PEG. The morphology of these nanoparticles, which was confirmed with microscopy and TEM, is spherical. The DNA release exhibited a first order kinetics. After 14 days, 15 of DNA per mg of nanoparticles have been released from the LTU2a nanoparticles. Furthermore, a successful uptake of LTU2a nanoparticles by the hepatic (LX2) cells has been confirmed with confocal laser microscope |
