| 초록 |
pdDronpa is a dimeric version of tetrameric Dronpa145N that is photo-switchable and photo-dissociable green fluorescent protein. It has faster kinetics of photo-switching than Dronpa145N by cyan light. In addition, it shows better dissociation and less aggregation which makes it suitable for photo-reversible hydrogel synthesis. We synthesized the hydrogel using 8-armed PEG and pdDronpa for light control. First, we confirmed that resulting hydrogel undergoes gel-sol transition by cyan light and violet light reversibly. Then, we introduced short DNA fragments into the hydrogel to encapsulate them. After cyan light illumination, released DNA were able to meet DNA chelating dye BOBO-3 which emits strong red fluorescence upon binding with DNA. |
