| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생물공학 |
| 제목 |
Construction of signal inverting amplifier with riboswitch and transcription repressor |
| 초록 |
Construction of complex circuits for practical applications requires numerous tunable genetic parts. However, the limited diversity and complicated tuning methods used for the input parts hinders the scalability of genetic circuits. Here, we developed RNA–protein hybrid input parts that combine a riboswitch and orthogonal transcriptional repressors. The hybrid inputs successfully regulated the transcription of an output in response to the input signal detected by the riboswitch and resulted in signal inversion because of the expression of transcriptional repressors. Dose–response parameters including fold-change and half-maximal effective concentration were easily modulated and amplified simply by changing the promoter strength. |
| 저자 |
노명현, 장성연, 장성호, 임현규, 정규열
|
| 소속 |
포항공과대 |
| 키워드 |
Genetic circuit; biosensor; coenzyme B12
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| E-Mail |
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