| 학회 | 한국화학공학회 |
| 학술대회 | 2005년 가을 (10/21 ~ 10/22, 인하대학교) |
| 권호 | 11권 2호, p.2049 |
| 발표분야 | 생물화공 |
| 제목 | Aggregation Behavior and Functions of Porcine Hepatocyte-HSC Co-spheroids |
| 초록 | There are many investigations concerning improvement of hepatocyte function and survival that cultured in vitro. These attempts include use of cytokines, growth factors and other medium supplements. However, one of the most promising techniques in retaining hepatocyte functions and viability is co-culturing with nonparenchymal liver cells such as sinusoidal endothelial cells, bile duct epithelial cells and hepatic stellate cells. Hepatic stellate cells (HSCs) are nonparenchymal cells with stellate morphology present in the perisinusoidal space of Disse and contain vitamin A-rich lipid droplets.1) When hepatocytes co-cultured with HSCs, hepatocytes expressed improved liver-specific functions like albumin secretion in monolayer culture.2) In this study, primary pig hepatocytes were co-cultured in suspension with pig HSCs to investigate functional activites of hepatocytes. Spheroids were embedded in alginate bead to prevent formation of over-size aggregates. And then activites of ammonia removal, urea synthesis and albumin secretion were investigated. References 1. R. Blomhoff, K. Wake, "Perisinusoidal stellate cells of the liver: important roles in retinol metabolism and fibrosis", FASEB J. 5 (1991) 271-277 2. S. Higashiyama, K. Yagi, "Maintenance of hepatocyte function in coculture with hepatic stellate cells", Biochem. Eng. J. 20 (2004) 113-118 |
| 저자 | 김 현, 박정극 |
| 소속 | 동국대 |
| 키워드 | hepatocyte; Hepatic stellate cell; co-culture |
| 원문파일 | 초록 보기 |
