| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 봄 (04/04 ~ 04/06, 대전컨벤션센터) |
| 권호 |
43권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
Development of Glycol chitosan-graft-low molecular weight polyethylenimine for biocompatible gene delivery carrier |
| 초록 |
A glycol chitosan-based polymer that spontaneously assembles with plasmid DNA into nanorods was evaluated as a non-viral vector for gene delivery. Glycol chitosan–methyl acrylate-polyethylenimine was synthesized by grafting polyethylenimine onto glycol chitosan via amidation after Michael addition using methyl acrylate. Gel retardation and PicoGreen assay experiments showed complete complex formation with plasmid DNA. GMP/pDNA complexes were characterized using biophysical techniques and were found to be positively charged rod-shape structures. Transfection efficiency and cytotoxicity was evaluated in HeLa, HEK293 and HepG2 cells, in comparison to high molecular weight polyethylenimine, a commonly used transfection reagent. Intracellular polymer uptake was compared and confirmed by confocal microscopy. The results demonstrate that GMP may serve as a promising vehicle for efficient gene delivery. |
| 저자 |
이영화, 최준식
|
| 소속 |
충남대 |
| 키워드 |
gene delivery; non-viral vector; glycol chitosan; polyethylenimine; cancer
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| E-Mail |
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