| 초록 |
Klebsiella oxytoca is one of the most promising 2,3-butanediol (2,3-BD) producers. In order to improve the metabolic performance of K. oxytoca as an efficient biofactory, it is necessary to assess its metabolic characteristics with a system-wide scope, and to optimize the metabolic pathways at a systems level. Provision of the complete genome sequence of K. oxytoca enabled the construction of genome-scale metabolic model of K. oxytoca and its in silico analyses. The stoichiometric model, KoxGSC1457, is composed of 1,457 reactions and 1,099 metabolites. Then, the model was applied to in silico analyses to understand the properties of K. oxytoca and also to improve its capabilities for 2,3-BD production according to genetic and environmental perturbations. [This work was supported by the Industrial Strategic Technology Development Program (No. 10050407) funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).] |
