| 학회 |
한국고분자학회 |
| 학술대회 |
2017년 봄 (04/05 ~ 04/07, 대전컨벤션센터) |
| 권호 |
42권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
Inducing Myogenic Differentiation of Human Mesenchymal Stem Cells through the 3D Co-culture with Myoblasts on Double-sided Scaffold |
| 초록 |
In the field of tissue engineering, there have been many attempts to induce differentiation of specific cell types from human mesenchymal stem cells (hMSC), and the advent of co-culture has definitely enhanced the overall differentiation process. In this study, we have combined the idea of co-culture materialized in 3D to provide an environment that is analogous to the in vivo system for cells. A fibrous scaffold is produced by patterning the polycaprolactone (PCL) electrospun nanofiber with poly(ethyleneglycol) (PEG) hydrogel in two ways vertically. Here, the nanofibrous scaffold accommodates 3D culture environment, and PEG functions as a support. In each side, hMSC and rodent C2C12 myoblasts are seeded separately to generate cellular interactions in between and enhanced differentiation of hMSC towards myoblasts with an aid of paracrine factors from C2C12. This dual-patterned hydrogel scaffold is predicted to be a practical platform in tissue engineering. |
| 저자 |
홍혜진, 한상원, 김민수, 조강희, 정윤아, 김경주, 노소영, 고원건
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| 소속 |
연세대 |
| 키워드 |
myogenesis; co-culture; MSC; C2C12; hydrogel scaffold
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| E-Mail |
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