| 초록 |
To prevent an immune response by host immunosystems, a semipermeable membrane can be used for a potential way of treating typeⅠdiabetes mellitus. In this study, to improve the diffusion property of the membranes, a centrifugal method was used to encapsulate the islets with very thin walls of agarose hydrogel. Various factors must be optimized to increase the efficiency of microencapsulation. The effects of agarose concentrations and centrifugal acceleration on the efficiency of microencapsulation were examined. Sephadex G-50 fine beads were employed as a model of the islets of Langerhans. The efficiency of microencapsulation increased with the increasing concentration alginate, and with decreasing centrifugal acceleration. The beads density should be less than 3,800 beads/ml to obtain microcapsules containing a single bead when a test-tube of 1.4 cm in diameter was used. The thickness of the agarose microcapsule was in the range of 5-10 m. It was concluded that the improved procedure could effectively enclose large number of islets in microcapsules with a very thin wall without contaminating of empty microcapsules. In addition, the insulin response of the microencapsulated islets was not significantly different than the naked islets in a static incubation secretagogue challenge. |
