| 초록 |
The three dimensional scaffolds have been widely used in tissue engineering, because they can guide cells to grow, synthesize extracellular matrix, and thus facilitate the formation of functional tissues and organs. However, the non-uniform cell distribution or cell necrosis due to the insufficient oxygen diffusion into the center of the scaffold have been considered as critical limitation for the formation of 3D tissues/organs. Although a variety of efforts to overcome this huddle, they are still remained as a huge challenge for tissue engineers. In this study, we developed a PFO-loaded hollow microparticles as a cell carrier which can allow continuous oxygen release from the emulsified PFO loaded in the microparticle for sufficient period, and thus provide appropriate environment for cell survival/growth even though in hypoxia. The viability and hypoxia-inducible gene expression of the cells seeded on the PFO-loaded hollow microparticle (in hypoxia) were investigated. |
