| 학회 | 한국공업화학회 |
| 학술대회 | 2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 | 25권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | 13C-MFA and Coculture of Escherichia coli for resveratrol production |
| 초록 | 13C-MFA (Metabolic Flux Analysis) is a technique for decoding fluxes of central carbon metabolism using a C source marked 13C. Provide a C source marked 13C in strain and measure the label information of the metabolite. It is then possible to track label information based on a model that decodes flux using specific enzymes in the strain. Resveratrol is a beneficial compound found in plants. It has the effects of cancer, antioxidants and life extension. Resveratrol can be created using Acyl-CoA in the EMP pathway and Tyrosine in the PP pathway. However, the road has a different temper and is difficult to turn into a single strain. Thus, an attempt was made to produce resveratrol using a coculture. In this study, two E. coli were designed to supply more resveratrol precursor based on previous studies. Then we will produce resveratrol with a coculture. Finally, using 13C-MFA to analyze the metabolic rate of coculture, and ways to increase resveratrol will be discussed. |
| 저자 | 김민선, 홍재승, 오민규 |
| 소속 | 고려대 |
| 키워드 | 13C-MFA; Escherichia coli; resveratrol |
