| 초록 |
This study was to develop nanoparticle which have a specific toxicity against the tumor cells. A cationic polymer/vitamin conjugate for preparation of the nanoparticles. The cationic polymer has cytotoxicity, but the nanoparticle from the conjugate slightly reduced the toxicity. However, there are still remained a toxicity. So that the nanoparticle was covered with biocompatible polysaccharide(BP) which can be easily degraded by an enzyme from tumor site. In this study, the cytotoxicity of nanoparticles was measured by MTT assay and compared to that without BP. In order to observed an interaction between the nanoparticle and the cells was used by confocal microscopy. Although the cell cytosol was perfectly stained by the labeling nanoparticles in the presence of the enzyme, it only stained in the cell membrane without the enzyme. The result indicated that BP can enhance the specificity of tumor site due to inhibit the internalization of the nanoparticle in the normal tissue. |
