| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 봄 (04/26 ~ 04/28, ICC 제주) |
| 권호 |
23권 1호, p.455 |
| 발표분야 |
생물화공 |
| 제목 |
Inducing Myogenic Differentiation of Human Mesenchymal Stem Cells through the Co-culture in 3D with Myoblasts on Double-sided Scaffold |
| 초록 |
In the field of tissue engineering, there have been many attempts to induce differentiation of specific cell types from human mesenchymal stem cells (hMSC), and the advent of co-culture has definitely enhanced the overall differentiation process. In this study, we have combined the idea of co-culture materialized in 3D to provide an environment that is analogous to the in vivo system for cells. A fibrous scaffold is produced by patterning the polycaprolactone (PCL) electrospun nanofiber with poly(ethyleneglycol) (PEG) hydrogel in two ways vertically. Here, the nanofibrous scaffold accommodates 3D culture environment, and PEG functions as a support. In each side, hMSC and rodent C2C12 myoblasts are seeded separately to generate cellular interactions in between and enhanced differentiation of hMSC towards myoblasts with an aid of paracrine factors from C2C12. This dual-patterned hydrogel scaffold is predicted to be a practical platform in tissue engineering. |
| 저자 |
홍혜진, 김민수, 조강희, 유병용, 김경주, 노소영, 한상원, 고원건
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| 소속 |
연세대 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
