| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Interaction of Cells with Hydroxyapatite Formed on PLLA Films and Scaffolds for Tissue Engineering |
| 초록 | Reconstruction of skeletal defects represents a major clinical challenge with over 1 million bone grafting procedures performed each year. Hydroxyapatite (HA) exhibits unique advantages when used in bone reconstruction because of its similar chemical structure as compared to the inorganic composition of human bone. HA formation on porous polymer scaffolds for tissue regeneration may demonstrate the enhanced osteoconductivity. In this study, the HA formation and characteristics on surface-modified poly(L-lactic acid) (PLLA) films and scaffolds were studied. Biodegradable nonporous PLLA films and dual pore scaffolds were prepared by using a solvent casting technique and both a gas foaming and phase-separation methods. The obtained hydrophobic PLLA film and dual pore scaffolds were activated by using Ar/acrylic acid (AA) plasma treatment for hydrophilic surface. The PLLA-PAA surfaces having carboxylic acid were immersed in simulated body fluid (SBF)1,2 for 30 days to yield HA formed-PLLA scaffold (PLLA-PAA-HA). The surface properties of various PLLA films and porous scaffolds were measured by ATR-FTIR, SEM, contact angle, XRD, ESCA, and EDS. In addition, the adhesion and proliferation of cells on some HA formed-scaffolds were evaluated. From SEM observation, the surface morphologies were unusually different with time (Fig. 1). The surfaces of the films were more hydrophilic after AA and HA grafting. The adhesion order of cells (fibroblast, chondrocyte, and osteoblast) to the surfaces was PLLA control ＜ PLLA-PAA ＜ PLLA-PAA-HA. Therefore, these biodegradable porous PLLA-PAA-HA scaffolds are useful for intelligent matrix to regenerate artificial tissues and organs. Fig. 1. SEM morphology of various modified PLLA film surfaces. References 1. R. Zhang and P. X. Ma, J. Biomed. Mater. Res., 45, 285 (1999). 2. X. Yuan, A.F.T. Mak, and J. Li, J. Biomed. Mater. Res., 57, 140 (2001). |
| 저자 | 정현정1, 안광덕1, 안동준2, 한동근1 |
| 소속 | 1한국과학기술(연), 2고려대 |
| 키워드 | PLLA scaffolds; Hydroxyapatite; Tissue engineering; Cells |
