| 초록 |
Advances in recombinant DNA techniques allow practical production of recombinant proteins from genetically engineered bacteria, yeast, or mammalian cells. Recombinant functional proteins including therapeutic proteins and biomaterials have been used for various biomedical applications. However, broader application of proteins has been hindered by the limited number of building blocks. In order to overcome this restriction, several techniques to incorporate non-natural amino acid building blocks into proteins were developed. Two biomedical applications of proteins containing non-natural amino acids will be presented. First, PEG molecules were conjugated into artificial extracellular matrix proteins containing non-natural amino acids via click chemistry. Second, site-specific fatty acid-bioconjugation to (therapeutic) proteins was achieved to prolong the serum half-life in vivo using site-specific incorporation of a reactive non-natural amino acid. |
