| 학회 |
한국고분자학회 |
| 학술대회 |
2013년 봄 (04/11 ~ 04/12, 대전컨벤션센터) |
| 권호 |
38권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Effect of Alginate and Demineralized Bone Particle Sponges with Chondrocytes for the Articular Cartilage Tissue Engineering |
| 초록 |
Alginate and demineralized bone particle (DBP) sponges were fabricated by 10, 20, 40 and 80 wt% alginate/DBP hybrid sponges for seeding cartilage cell. Cell proliferation activity was measured via MTT assay. Morphological observation, histology, biological assay for collagen type I, II and aggrecan, and PCR were performed at each time point 1, 2 and 3 weeks. The cell viability was better in 20 and 40 wt% alginate/DBP sponges than other wt% alginate/DBP sponges. Scanning electro microscope (SEM) results showed that more attach cell in the 20 and 40 wt% alginate/DBP sponges with the lapse of time. Dimethylmethylene Blue (DMMB) results showed that the high content of Glycosaminoglycan (GAG) and collagen levels at 20 and 40 wt% alginate/DBP sponges. This study suggested that alginate/DBP sponge may serve as a potential cell delivery vehicle and a structural basis for tissue engineered articular cartilage. This research was supported by WCU (R31-20029) and MBC (0405-B001-0204-0006) |
| 저자 |
오승창1, 김혜민1, 김아람1, 김경희1, 김영래1, 윤근호2, 이동원1, 강길선1
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| 소속 |
1전북대, 2가톨릭대 |
| 키워드 |
Alginate; Demineralized bone particles; Hybrid sponge; Chondrocytes
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| E-Mail |
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