| 초록 |
Hetero thin films,consisting of green fluorescent protein (GFP) and cytochrome c, was fabricated by self-assembly technique. Protein formation onto the substrate was performed by the self-assembly technique. In order to make molecular assembly onto the gold substrate, cytochrome c was adsorbed onto the 11-mercaptoundecanoic acid (11-MUA) by electrostatic attractive force. 11-MUA can be spontaneously adsorbed onto the gold substrate via thiol group, leaving its one end (carboxyl group) toward surface, and thereby providing the template for cytochrome c adsorption. GFP can also be adsorbed onto the cytochrome c surface by electrostatic attractive force. The surface structure of absorbed GFP film onto the cytochrome c layer was analyzed using atomic force microscope (AFM) and scanning tunneling microscopy (STM). |
