| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생체재료 |
| 제목 |
Development and Evaluation of bioreducible gene carrier for peptide/gene co-delivery |
| 초록 |
(KLA)4 peptide is well-known as pro-apoptotic peptide, which could lead to anticancer effect through mitochondria penetration into cancer cell. However, its utilization has been limited due to inefficient delivery in cancer cell. To solve these problems, bioreducible gene carrier (BGC) composed with (KLA)4 peptide, water-soluble chitosan (WC) and cystamine (Cys) was synthesized by coupling agent. The chemical structure was analyzed by 1H-NMR. BGC/gene complex was prepared by electrostatic charge interaction. Particle size, zeta potential and morphological property of the BGC/gene complexes were confirmed by DLS and TEM. Its binding affinity, DNase protection and releasing assay were carried out by gel retardation assay. Moreover, its transfection efficacy was evaluated in diverse cancer cell lines. These results suggest that BGC has potential ability as gene delivery carrier.
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| 저자 |
김준수1, 홍웅길1, 안준혁1, 정경원2, 박준규3, 심왕근1, 나재운1
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| 소속 |
1순천대, 2조선이공대, 3시지바이오 |
| 키워드 |
(KLA)4 peptide; Gene; Water-soluble chitosan; Bioreducible; Anticancer effect
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| E-Mail |
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