| 학회 | 한국화학공학회 |
| 학술대회 | 2009년 봄 (04/23 ~ 04/24, 광주 김대중컨벤션센터) |
| 권호 | 15권 1호, p.380 |
| 발표분야 | 생물화공 |
| 제목 | Protein-conjugated, glucose-sensitive surface using fluorescent dendrimer porphyrin |
| 초록 | A multi-functional dendrimer-coated surface has been prepared foreffective protein immobilization anddetection of protein activity.Silicon surface was first modified with positively-charged amine groups using 3-aminopropyltriethoxysilane(APTES), and then coated withdendrimer porphyrin by electrostatic interaction. Fluorescence andAFM studies showed that dendrimer was homogeneously coated onAPTES-modifiedsurface as dome-shaped features which were protruded 1.0~2.5 nm abovesurface and had diameters ranged50~100 nm.Dendrimer-modified surface showed greater protein capacity, compared withAPTES-modified surface without dendrimer, andprotein activity was higher by a factor of two.Usingfluorescent property ofporphyrin,relative amounts of dendrimer and activities of proteins immobilized on the dendrimer-coated surface were examined by fluorescence microscopy.For example, glucose oxidase (GOX)-mediated glucose oxidation quenched fluorescence emission fromfocal porphyrin core through a peroxidase-coupled system and from the quantitative relationship between quenching and glucose concentration, we could characterize the GOX-catalyzed reaction. |
| 저자 | 이 열, 김종수, 김수현, 장우동, 고원건 |
| 소속 | 연세대 |
| 키워드 | dendrimer porphyrin; surface modification; protein activity |
| 원문파일 | 초록 보기 |
