| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
22권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Biosynthesis of cadaverine from galactose by reconstruction of metabolic pathways in Escherichia coli |
| 초록 |
As an alternative way to replace polyamides from the petroleum-based process, biological fermentation of cadaverine has become one of solutions. Although cadaverine has to be fermented with high yield and productivity from diverse sugars rich in biomass for an ideal production, however, most microorganisms are not feasible to metabolize other biomass-derived sugars as efficiently as glucose. Because of it, low growth rate and carbon flux for the production has become the problem. Thus, we engineered Escherichia coli to produce cadaverine from galactose, a promising future feedstock. For doing this, genes related in the production of cadaverine were overexpressed through synthetic expression cassettes. In addition, we inactivated the feedback inhibition and degradation/re-uptake pathways. Finally, the resultant strain produced 8.80 g/L cadaverine with high yield (0.170 g/g) and productivity (0.293 g/L/h). These values were similar to or better than the previous glucose fermentation. |
| 저자 |
예대열1, 차상학1, 김동훈1, 임현규1, 양진아2, 서상우2, 정규열1
|
| 소속 |
1포항공과대, 2서울대 |
| 키워드 |
Cadaverine; Galactose; Carbon flux
|
| E-Mail |
|
