| 초록 |
Hyaluronic acid (HA) is a natural polymer composed of glucuronic acid and N-acetylglucosamine and it has excellent biocompatibility, biodegradability, biological activity, and viscoelastic property. But HA has a relatively low cell growth rate because of its poor cell adhesion. So, we added fragmented polycaprolactone (PCL) electrospun nanofiber to the HA so that the cells could adhere to the fragmented PCL nanofiber and grow. In this study, we fabricated a composite hydrogel scaffold. PCL nanofibers made by electrospinning were hydrolyzed into fragmented nanofibers and dispersed in a HA solution to generate a complicated ECM-mimicking environment. After the preparation of a composite hydrogel scaffold of encapsulated fibroblasts, mechanical properties and cells behaviors were compared with HA hydrogel without fragmented PCL nanofibers. The mechanical properties were measured using a rheometer. We evaluated a Live/Dead assay, GAGs, and collagen assay. |
