| 초록 |
5CB microdroplets coated with PAA-b-LCP and PNIPAM-b-LCP were successfully made via microfluidics. Their functionalized surfaces at the water/5CB interface were examined for protein detection which could be easily visualized with an optical microscope under crossed polarizers by the radial to bipolar configurational change. The GOx and urease were immobilized on the PAA chains for glucose and ureas detection, respectively, by a EDC/NHS reaction. The H+ and OH- ions released by the GOx and urease reactions of glucose and urea, respectively were detected by the radial to bipolar (or vice versa) configurational change of 5CB droplets. This study demonstrated a simple approach for the development of a liquid crystal-based sensor for label-free detection of proteins, glucose, and urea which may have diverse applications in various biomedical fields |
