| 학회 | 한국공업화학회 |
| 학술대회 | 2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 | 26권 1호 |
| 발표분야 | 포스터-생체재료 |
| 제목 | Optimization of In Vitro Transcribed (IVT) mRNA Synthesis for Improved Translation Efficiency. |
| 초록 | The prospect of IVT mRNA therapeutics can go beyond the limits of plasmid DNA for target protein treatments. It is receiving a lot of attention in the field of gene therapy and regenerative medicine. The IVT mRNA template consists of a T7 promoter, a 5′UTR, an ORF, and a 3′UTR. Cap analogues and modified rNTP are mainly used toincrease the translation efficiency of IVT mRNA and modulating the immune response. In addition, removal of dsRNA fragment generated during the IVT process is recommended through various purification methods. We designed GFP and Luc reporter gene constructs for IVT mRNA synthesis and optimized the IVT condition. In our study, modified mRNA prepared with pseudo-UTP and AG-clean cap showed enhanced GFP and Luc expression as compared to the native mRNA. It was confirmed that the synthesis yield and translation efficiency were significantly different depending on the cap analogue. |
| 저자 | 김은빈, 장보라, 윤지연, 이혁진 |
| 소속 | 이화여자대 |
| 키워드 | In vitro transcription; Synthetic modified mRNA; Gene therapy |
