| 학회 | 한국공업화학회 |
| 학술대회 | 2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 | 26권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | 3-hydroxypropionic acid export phenotypes screening by synthetic cellular communication |
| 초록 | Although cellular export is important in industrialbiotechnology, its assessment is difficult due to the lack of efficientanalytical methods. This study describes a synthetic cellularcommunication-based microfluidic platform for screening improved exportphenotypes. Metabolite-secreting cells were compartmentalized in droplets, withsensing cells equipped with a genetic circuit that converts the export levelinto an easily detectable signal. This platform was applied to identify Escherichiacoli genes that enhance the exportation of 3-hydroxypropionic acid, anindustry-relevant platform chemical. Resultantly, two genes (gene X, gene Y)found by this platform enhance the secretion of 3-hydroxypropionic acid and itsproduction. Given the increasing design capability for chemical-detectingcells, this platform has considerable potential in identifying efflux pumps formany important chemicals. |
| 저자 | 김승진, 정규열 |
| 소속 | POSTECH |
| 키워드 | 3HP; Metabolic Engineering |
