| 초록 |
Numerous investigations have focused on the development of functional protein chips as diagnostic tools in medicine and biotechnology. In systems biology, functional protein chips can play an important role for the in vitro optimization of metabolic pathways and even the cell-free production of biomolecules. To accommodate large number of proteins and minimize preparation time using genomic information only, a simple method avoiding long and expensive protein purification steps is required. In this research, a new functional protein chip employing RNA-protein fusion molecules was investigated. A sequential enzymatic reaction catalyzed by nucleoside diphosphate kinase (NDK) and luciferase on a 96-well microplate was demonstrated as a potential for a functional protein chip. Subsequently, a combinatorial pathway optimization for trehalose synthesis was conducted. The results suggest that the fusion molecules immobilized by hybridization of mRNA end and cDNA retained this method allows the quick investigation of numerous combinations of enzymes participating in a conversion pathway for the determination of optimal conversion conditions in complex transformations. |
