| 초록 |
RNA interference (RNAi) using small interfering RNA (siRNA, a double-stranded RNA molecule having 21 to 23 base-pairs) has recently provided a powerful tool for silencing a target gene in gene therapy. RNAi is the process of inducing highly sequence-specific degradation of homologous mRNA by double-stranded RNA (dsRNA). The process of RNAi is very useful for genetic analysis and is likely to become a potent therapeutic approach for gene silencing. Currently, several groups have used siRNA for treating infectious diseases and other types of illnesses mediated by variant gene expression like common types of cancers. Even though siRNAs are highlighted as gene therapeutics, the hurdles related to their delivery, such as rapid degradation, poor intracellular uptake, and nonspecific biodistribution, are still remained. To overcome these disadvantages, it is necessary to develop an appropriate carrier system. In this work, we studied to develop effective siRNA delivery systems by using PEGylated synthetic siRNA or modified cationic polymers with a cancer target ligand. These siRNA delivery systems showed a significant decrease in the expression level of the target gene in target cells. |
