| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 봄 (04/08 ~ 04/09, 대전컨벤션센터) |
| 권호 |
35권 1호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Fabrication of protein array on the micropatterned substrate using dendrimer porphyrin and layer-by-layer (LbL) method |
| 초록 |
A dendrimer-coated surface is one of the promising candidates for the design of effective microarray, because it provides a 3-D architecture with numerous functional groups to immobilize proteins, and can minimize protein denature due to flexible nature of branched arm. Here, to prepare protein microarray with high sensitivity and stability, a dendrimer porphyrin (DP) with 32 carboxyl groups on the periphery was used as a potential building block for immobilizing proteins. By combining photolithography, LbL self assembly and spin self-assembly technique, micropatterned (PEI/PSS)40PEI/DPs multilayer structure with size of 50 um was successfully constructed. Feasibility of immobilizing protein onto DP was confirmed by visualizing localization and patterning of FITC-conjugated casein. Finally, immunoassay was performed using antibody, and it was found that 0.1 20 ug/ml of target antigen could be successfully detected with enhanced fluorescence signal and sensitivity. |
| 저자 |
손경진, 이열, 박상필, 장은지, 이현종, 남승희, 김주호, 장우동, 고원건
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| 소속 |
연세대 |
| 키워드 |
protein array; layer-by-layer deposition; photolithography; immunoassay; dendrimer
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| E-Mail |
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