| 초록 |
In this study, we tried to decompose synthetic industrial dyes using Bacillus subtilis spore, which has CotA spore coat protein known as having multi-copper oxidase (laccase) activity. We confirmed the decolorization rate of Acid green 25 by 20 % in 5 hrs using a wild-type Bacillus subtilis spore. To express CotA protein on the surface of the spores, anchoring motives CotE was used. And we confirmed the decolorization rate of Acid green 25 by 56% in 5 hrs using a recombinant Bacillus subtilis spore. His6-tag was added at the C-terminal of target protein, CotA. The spore surface expression of target protein, CotA, was confirmed by flow cytometry using FITC labelled anti-His6 antibody. |
