| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 봄 (04/23 ~ 04/25, 제주ICC) |
| 권호 |
14권 1호, p.441 |
| 발표분야 |
생물화공 |
| 제목 |
Selective Aggregation Mechanism of Unmodified Gold Nanoparticles in Detection of Single Nucleotide Polymorphism |
| 초록 |
Detection of single point mutation based on the hybridization of oligonucleotides was performed using unmodified gold nanoparticles. The sequences of oligonucleotides were designed to detect the metastatic efficiency modifier signal-induced proliferation-associated gene 1 (Sipa1). The detection step was monitored using UV-vis absorption spectroscopy, quasi-elastic light scattering (QELS), and zeta potential measurement. We observed that addition of DNAs into the suspension of unmodified gold nanoparticles could substantially aggregate the gold nanoparticles and change the color of solution. By changing the salt concentration in the presence of a phosphate buffer solution, we were able to selectively aggregate gold nanoparticles for the perfectly-matched DNA, which enabled a detection of perfectly-matched DNA from the single point-mutated one. Our results indicate that a change in the electrostatic interaction is responsible for the selective aggregation of gold nanoparticles upon the addition of DNA. This suggests a novel design principle for a rapid detection of the DNA sequence by controlling the electrostatic interactions between gold nanoparticles. |
| 저자 |
이윤희1, 조경남1, 이강택1, 주상우2, 류판동3, 이소영3
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| 소속 |
1연세대, 2숭실대, 3서울대 |
| 키워드 |
DNA; hybridization; single nucleotide; polymorphism; gold nanoparticle; aggregation
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| E-Mail |
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| 원문파일 |
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